Quantifying length-dependent DNA end-binding by nucleoproteins
نویسندگان
چکیده
منابع مشابه
Structural mechanism of ATP-dependent DNA binding and DNA end bridging by eukaryotic Rad50.
The Mre11-Rad50-Nbs1 (MRN) complex is a central factor in the repair of DNA double-strand breaks (DSBs). The ATP-dependent mechanisms of how MRN detects and endonucleolytically processes DNA ends for the repair by microhomology-mediated end-joining or further resection in homologous recombination are still unclear. Here, we report the crystal structures of the ATPγS-bound dimer of the Rad50(NBD...
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The FANCI-FANCD2 (I-D) complex is considered to work with RAD51 to protect the damaged DNA in the stalled replication fork. However, the means by which this DNA protection is accomplished have remained elusive. In the present study, we found that the I-D complex directly binds to RAD51, and stabilizes the RAD51-DNA filament. Unexpectedly, the DNA binding activity of FANCI, but not FANCD2, is ex...
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ژورنال
عنوان ژورنال: Biophysical Chemistry
سال: 2019
ISSN: 0301-4622
DOI: 10.1016/j.bpc.2019.106177